Part:BBa_I742110:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I742110

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

Team TU Munich 2012 used this part for expression of limonenesynthase and added a yeast consensus sequence for improved expression as well as a Strep-Tag for simple purification or detection via western blot. See BBa_K801060. Since the DNA of BBa_I742110 is not available and we submitted our BioBrick BBa_K801060 we improved this part by making limonene synthase physically available in the registry.

User Reviews

UNIQf6843e8939046d7a-partinfo-00000002-QINU

No review score entered. iGEM TU Munich 2012

BBa_K118025 is based on this part. British Columbia statet on the experience page that they could not detect the expression of Limonene-Synthase or the production of limonene. We report that we were not able to express Limonene-Synthase, but also that we showed its functionality both in vitro and in vivo. See our Biobrick BBa_K801060.

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